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KMID : 0378019750180081036
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New Medical Journal
1975 Volume.18 No. 8 p.1036 ~ p.1042
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Net Urea Production in the Isolated Perfused Cirrhotic Liver of Rats
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Abstract
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Albino Sprague-Dawley rats were fed choline deficient, low protein diet for 142 to 229 days, and was cirrhosis produced. Glutamine and glucagon effects on urea production were observed in the isolated perfused liver. Perfusion was carried out by the method of Miller, and after 2 hours of perfusion glutamine oar glucagon was added for 4 hours at a constant rate with an infusion pump. Amounts of infusion were 10 pg per hour for glucagon and 0.25 or 0.5 mmole per hour for glutamine. The results were as follows:
1) Liver weight-25.5g, hepatic total lipids-28.7%, and bile flow during 6 hours perfusion-2.2ml were shown for the cirrhotic liver.
2) Although amino acid nitrogen accumulation was high, urea production and expired respiratory ´?CO2 were less in the cirrhotic liver than in the control.
3) Glutamine increased urea production, and glutamine may play a role of not only substrate of urea but also others in the cirrhotic liver.
4) Glucagon did not increase urea production in the cirrhotic liver.
5) Urea excretion in 24 hours urine was 131.8mg in the cirrhotic rats and 184.4mg in the controls. The amounts were higher compared to urea production by the isolated perfused liver of cirrhotic rats. Negative nitrogen balance was showed in the cirrhotic rats.
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